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. 2012 May 14;287(30):25086–25097. doi: 10.1074/jbc.M112.340521

FIGURE 5.

FIGURE 5.

Cardiac mitochondrial substrate selectivity and bioenergetic efficiency are driven by cardiolipin synthase transgenic expression. High resolution respirometry demonstrated substrate-selective control of respiration utilizing pyruvate (Pyr) (A), palmitoyl-l-carnitine (Palm-Carn) (B), glutamate (Glut) (C), and pyruvate and glutamate (Pyr Glut) (D) through various respiratory and electron transport chain control states (E). F, comparative analysis of substrate control ratios revealed an overall decrease in substrate-stimulated respiration. However, cardiac mitochondria from CLS transgenic mice utilized less oxygen to generate an equivalent amount of ATP (G), demonstrating a high degree of coupling per molecule of oxygen consumed. FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone; AA, antimycin A; Black bar, wild type mice; white bar, CLS transgenic mice; *, p < 0.05; **, p < 0.01. Error bars, S.E.