FIGURE 4.

Cell-type specificity of hGH-N expression is maintained in the hGH(ΔHSII) transgenic pituitary. Disaggregated pituitary cells from two hGH/P1(ΔHSII) transgenic lines were stained with antibodies specifically recognizing mouse (red) or human (green) growth hormone. Nuclei were also visualized with TO-PRO-3 iodide (blue). In an analysis of >100 cells from each of two transgenic lines, no cells were found to be positive for hGH-N in the absence of the mouse GH. All cells shown in the sample figure express both proteins. Thus, the deletion of HSII did not alter the strict somatotrope specificity of hGH expression.