FIGURE 7.

Deletion of HSII did not alter histone H4 acetylation at HSI or the hGH-N promoter. A, ChIP analysis of histone H4 acetylation in pituitary chromatin from the hGH/P1 and hGH/P1(ΔHSII) transgenic lines. ChIP assays were performed on pituitary chromatin from animals doubly transgenic for either hGH/P1 or hGH/P1(ΔHSII) and the hGRF transgene. The fold-enrichment was calculated from the antibody-bound fraction over IgG-bound background and normalized to the signal at the inactive promoter of myoD. Bar heights represent averages. Two independent lines were analyzed; asterisks indicate values for each line. HSI and hGH-Np below the graph indicate the respective primer locations. B, representative histone H4 acetylation ChIP assay. An ethidium bromide-stained agarose gel is shown. Input dilutions are serially 10-fold. The control IgG antibody recognizes total rabbit immunoglobulin G.