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Gid5 is the adaptor molecule for Gid4. A, shown are co-IPs between Gid4 and Gid5 in the wild type background (WT) and the Δgid strain as described in Fig. 3. Cells were grown overnight in YPE and harvested 20 min after the addition of glucose. B, shown are co-IPs between Gid1 and Gid4 in distinct GID deletion strains. Cells were grown overnight in YPE and harvested 20 min after the addition of glucose. Gid5 was detected with polyclonal antiserum.
