TABLE 1.
Pellino 2 is critical for IL-1- and LPS-mediated mRNA stabilization.
mRNA decay rates and half-lives of human IL-8, mouse KC, TNFα, and IL-6 mRNA were measured from the experiments done in Fig. 6, B and E.
| mRNA | shRNA | Ki | t½ | P-valuea |
|---|---|---|---|---|
| h−1 | min | |||
| Human IL-8 | Scrambled | −0.104 ± 0.09 | 472 | <0.01 |
| Peli2 KD | −0.173 ± 0.06 | 291 | ||
| Mouse KC | Scrambled | −0.104 ± 0.20 | 426 | <0.01 |
| Peli2 KD | −0.319 ± 0.11 | 187 | ||
| Mouse TNFα | Scrambled | −0.458 ± 0.08 | 135 | 0.08 |
| Peli2 KD | −0.57 ± 0.13 | 113 | ||
| Mouse IL-6 | Scrambled | −0.114 ± 0.23 | 375 | <0.05 |
| Peli2 KD | −0.22 ± 0.14 | 235 |
a Two-way ANOVA analysis demostrated a statistically difference (P-value) between the curves (shown in Fig. 6, B and E) for Scrambled and Peli2 KD cells.