FIGURE 1.

Cellular localization of Chk1. A, schematic diagram of human Chk1 and generation of deletion mutation strategy. SQ, Ser/Gln cluster; CM, conserved motif; FL, full-length (476 amino acids); N, N terminus (amino acids 1–264); C, C terminus (amino acids 265–476). B, U2-OS cells grown on glass coverslips were transfected with Myc-tagged vectors expressing full-length Chk1 or mutants for 48 h, fixed, stained with anti-Myc antibodies, and visualized using a fluorescence microscope (63× oil lens).