FIGURE 7.
The complex modulation of the signaling of five mutant 5-HT3A receptors exerted by PU02. Concentration-response curves for 5-HT in the absence or presence of five different concentrations of PU02 at tsA201 cells expressing WT, S248V, T294A, T294L, T294Q, and G306A 5-HT3A receptors in the FMP assay. The experiments were performed as described under “Experimental Procedures,” and the results shown represent data ± S.D. (error bars) of duplicate determinations from representative experiments (n = 3–4). FU, fluorescence units.