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. 2012 Apr 25;287(30):25325–25334. doi: 10.1074/jbc.M112.370809

FIGURE 4.

FIGURE 4.

A, PANC1 cells were transfected with two shRNA vectors targeting AKT1 (shAKT1 (1) and shAKT1 (2)), AKT2 (shAKT2 (1) and shAKT2 (2)), AKT3 (shAKT3 (1) and shAKT3 (2)), and the non-targeting control vector (NT). VMP1 mRNA expression levels were determined after 72 h by real-time RT-PCR. The effect of knockdown on AKT1, AKT2, and AKT3 expression was examined by RT-PCR. B, PANC1 and HeLa cells were transfected with the VMP1-luciferase reporter construct and a constitutively active PI3K construct (caPI3K) or a constitutively active AKT1 construct (caAKT1) or the empty vector (control). After 24 h, VMP1 reporter activity was determined by luciferase assay. C, RNA was isolated from HeLa cells transfected with the construct caAKT1 or the control vector, and VMP1 mRNA expression was analyzed by real-time RT-PCR. D, PANC1 cells were transfected with the VMP1-luciferase reporter, a shKRAS construct (shKRAS) or NT control vector, and the constitutively active AKT1. After 72 h, reporter activity was determined by luciferase assay. In all panels, bars represent the mean of triplicate experimental wells ± S.E.