TABLE 1.

Specific activity and kinetics of Cel5B_Dtu and mutant D14N

CMC, carboxymethyl cellulose; S.A., specific activity; CGM, carob galactomannan.

Substrate	Activity parameters	Cel5B_Dtu		Improvement
		WT	D14N
				%
CMC	S.A. (units × mg−1 protein)	28.89 ± 0.96	50.03 ± 0.97	73.17
	kcat (s−1)	408.19	550.66	34.90
	Km (mg × ml−1)	24.02	11.76	104.25
	kcat/Km (ml × mg−1 × s−1)	17.00	46.81	175.35
CGM	S.A. (units × mg−1 protein)	2.11 ± 0.03	8.83 ± 0.36	318.48
	kcat (s−1)	68.25	71.82	5.24
	Km (mg × ml−1)	11.57	0.72	1506.94
	kcat/Km (ml × mg−1·s−1)	5.90	99.89	1593.05