Figure 2. Genetic inactivation of AXL does not affect ovarian tumor cell proliferation in vitro or subcutaneous growth in vivo.
A. Western blot analysis of AXL expression in a panel of human ovarian cancer cell lines. B. Western blot analysis of AXL expression in SKOV3ip.1 and OVCAR-8 cells stably transfected with shRNA targeting sequences for scramble control (shSCRM) or AXL (shAXL). Heat shock protein 70 (Hsp70) was used as a protein loading control. C. Cellular growth curves for SKOV3ip.1 (left), and OVCAR-8 (right) shSCRM) and shAXL cells (n = 3). Error bars represent the S.E.M. D. Average tumor volumes of subcutaneous SKOV3ip.1 tumors (n = 4 mice per group) grown over a 48-day time course. Error bars represent the S.E.M.. All full-length blots are presented this figure are in Supplementary Figure 7.