Skip to main content
. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Cell. 2012 Mar 30;149(1):63–74. doi: 10.1016/j.cell.2012.01.051

Figure 1. Generation of mice deficient for VHL or HIF-1 and HIF-2 in osteoblasts.

Figure 1

A–C. ROSA-LacZ reporter expression of Osterix-Cre (A–B) in 8-week-old mouse tibia. Arrows in (A) point to osteoblasts at the endosteal bone surface and embedded in cortical bone with B-galactosidase activity. B. Shows the absence of detectable B-galactosidase activity in ROSA-LacZ mice without OSX-Cre. C. Arrows point to subset of hypertrophic chondrocytes expressing B-galactosidase activity. D. Efficient recombination of the VHL, HIF-1, and HIF-2 alleles in the bone of Osterix-Cre mutant mice. PCR analysis of genomic DNA isolated from bone of 1) OSX-Cre control, 2) OSX-VHL, 3) OSX-Cre control, and 4) OSX-HIF-1/HIF-2 mice. Abbreviations: conditional allele, 2-lox; recombined allele, 1-lox; and wild-type allele, WT. E. Immunohistochemical analysis of HIF-1 (top) and HIF-2 (bottom) expression in OSX-Cre tibias. Arrows point to osteoblasts expressing HIF-1 or HIF-2.