Skip to main content
. 2012 Jun 29;14(8):994–1006. doi: 10.1093/neuonc/nos138

Fig. 4.

Fig. 4.

RNAi-mediated suppression of PS2-inhibited growth of U251 cells in vitro. (A) Cell growth curve. Parental U251 cells, vector control U251 cells (U251-NC), and PS2 shRNA-stably transfected cells (U251-S and U251-Smix) were seeded onto 96-well tissue culture plates. Cell viability was measured using the MTT assay. Cell growth curves were determined by reading the absorbance at 490 nm on a multiscanner reader. (B) Cell cycle distribution. Parental U251 cells, vector control U251 cells (U251-NC), and PS2 shRNA-stably transfected cells (U251-S and U251-Smix) were stained with propidium iodide. The DNA content of the cells was analyzed by FACS. (C) The differences in cell cycle distribution of parental U251 cells, vector control U251 cells (U251-NC), and PS2 shRNA-stably transfected cells (U251-S and U251-Smix). Cells in G0/G1, S, and G2/M of the cell cycle were sorted based on DNA content. Cells (10 000) were sorted using flow cytometry and analyzed. (D) Cell apoptosis in shRNA-stably transfected U251 cells. Parental U251 cells, vector control U251 cells (U251-NC), and PS2 shRNA-stably cells (U251-S and U251-Smix) were stained by annexinV/7-ADD and analyzed by FACS to detect apoptosis. Values are shown as the mean ± SD of 3 independent experiments.