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. Author manuscript; available in PMC: 2012 Jul 30.
Published in final edited form as: Methods Mol Biol. 2011;793:273–296. doi: 10.1007/978-1-61779-328-8_18

Fig. 1.

Fig. 1

In-gel assay for proteasome activity and assembly in rat E18 primary cortical neuronal cultures. Crude extracts were prepared from control cultures (−) or cultures treated with 15 μM PGJ2 for 16 h (+). Cleared lysates (40 μg/sample) were subjected to nondenaturing gel electrophoresis. In (a) the proteasomal chymotrypsin-like activity was measured with Suc-LLVY-AMC by the in-gel assay. In (b and c) 26S and 20S proteasomes were detected by immunoblotting with an anti-β5 antibody (b), a subunit of the core proteasome particle (20S), and with the anti-Rpt6 antibody (c), an ATPase subunit of the 19S regulatory particle. Proteasomal 26S (two caps and one cap) and 20S forms are indicated by on the left.