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Proteasome activities in SK-N-SH cells. Cells were treated for 24 h with DMSO (control, vehicle) or epoxomicin (25 nM). Proteasome activities were measured in cleared supernatants obtained from total cell homogenates (50 μg of protein/sample). Peptidase activities were assayed colorimetrically after a 24-h incubation at 37°C. The chymotrypsin-like activity was measured with Suc-LLVY-AMC, the trypsin-like activity with Z-GGR-βNA, and the caspase-like activity with Z-LLE-βNA.
