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. Author manuscript; available in PMC: 2012 Jul 30.
Published in final edited form as: Methods Mol Biol. 2011;793:273–296. doi: 10.1007/978-1-61779-328-8_18

Fig. 5.

Fig. 5

Proteasome subunit levels in rat E18 primary cortical neuronal cultures. Aliquots of the supernatant (cleared lysate) and pellet fractions obtained from samples prepared for the “in-gel” assay (see Fig. 1) were run on SDS-PAGE (10% gel) followed by immunobloting with the same antibodies listed in Fig. 1 as well as with anti-β-actin. 40 μg of protein were loaded per lane.