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Accumulation of ubiquitinated proteins in rat E18 primary cortical neuronal cultures. Cells were treated for 24 h with DMSO [control, vehicle (−)] or with 20 μM PGJ2 (+). Total cell extracts were subjected to western blot analysis (8% gel) to detect ubiquitinated proteins (40 μg of protein/lane). Equal protein loading was demonstrated by probing the immunoblots with the anti-β-actin antibody.
