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. Author manuscript; available in PMC: 2012 Jul 30.
Published in final edited form as: Methods Mol Biol. 2011;793:273–296. doi: 10.1007/978-1-61779-328-8_18

Fig. 7.

Fig. 7

Filter trap assay to measure ubiquitin-protein aggregates in rat E18 primary cortical neuronal cultures. (a) In a preliminary test, waterproof drawing ink (100 μl per well) was applied to the nitrocellulose membrane to assess flow through each well. If vacuum is properly equilibrated, dye dots corresponding to each well should appear round and neat on the nitrocellulose membrane. (b) Influence of membrane composition and porosity on detection of ubiquitin-protein aggregates. Cells were treated for 16 h with DMSO [control, vehicle (0)] or with 15 μM or 20 μM PGJ2. Cell extracts were subjected to the filter trap assay to detect ubiquitin-protein aggregates (100 μg of protein/sample). Nitrocellulose-yielded optimal assay sensitivity relative to polyvinylidene fluoride (PVDF) or cellulose acetate.