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. Author manuscript; available in PMC: 2012 Jul 30.
Published in final edited form as: Methods Mol Biol. 2011;793:273–296. doi: 10.1007/978-1-61779-328-8_18

Fig. 8.

Fig. 8

Immunofluorescence detection of ubiquitin-protein aggregates in rat E18 primary cortical neuronal cultures. Ubiquitinated proteins and βIII-tubulin immunofluorescence staining of cortical cultures treated with DMSO (top 4 panels) or 15 μM PGJ2 for 16 h (bottom 4 panels). Nuclei are stained with DAPI. Large arrows point to protein aggregates and small arrows to dystrophic neurites. Scale bar = 10 μm.