Table 1.

Native gel solutions

Gel components	3% (top)	4% (middle)	5% (bottom)
Layer height (cassette 8 × 8 cm)	3 cm	3 cm	2 cm
30% acrylamide/Bis solution, 29:1	1,260 μL	1,680 μL	2,100 μL
Rhinohide polyacrylamide gel strengthener	240 μL	320 μL	400 μL
Ammonium persulfate (1.5%, w/v)	600 μL	600 μL	600 μL
Gel buffer (complete with ATP and DTT)	9.888 mL	9.388 mL	8.888 mL
TEMED	12 μL	12 μL	12 μL
Total volume	12 mL	12 mL	12 mL