Table 2.

SDS-polyacrylamide minigel solutions

Gel components	8%	10%	Stacking
30% acrylamide/Bis solution, 29:1	2.7 mL	3.4 mL	1.03 mL
Resolving buffer	1.3 mL	1.3 mL	None
Stacking buffer	None	None	2.07 mL
Ammonium persulfate (1.5%, w/v)	515 μL	515 μL	0.41 mL
Water	5.37 mL	4.67 mL	3.6 mL
SDS (10%, m/v)	105 μL	105 μL	0.83 mL
TEMED	10 μL	10 μL	6 μL
Total volume	10 mL	10 mL	8 mL