FIGURE 8:

Dynamin recruits Myo1E independent of its proline-rich domain. (A, B) Cotransfection of Mito-GFP-Myo1E and dynamin-mCherry shows that the latter is not recruited to the mitochondria. (C) The merge of A and B. (D–F) Cotransfection of Mito-GFP-dynamin and Myo1E-dTomato shows that Myo1E-dTomato is efficiently recruited to the GFP-dynamin–decorated mitochondria. (F) The merge of D and E. (G–I) High magnification of D–F showing the colocalization of GFP-dynamin–decorated mitochondria and Myo1E-dTomato. (I) The merge of G and H. (J–L) Cotransfection of Mito-GFP–dynamin ΔPRD and Myo1E-dTomato shows efficient recruitment of Myo1E-dTomato to the mitochondria. (L) The merge of J and K. (M–O) High magnification of J–L showing the colocalization of GFP-dynamin ΔPRD–decorated mitochondria and Myo1E-dTomato. (O) The merge of M and N. Bars, 10 μm.