Figure 7. A3H HapIIΔ15N has little RNA binding affinity.

A) To compare the RNA binding activity between A3H HapII and HapI, 293T cells were transfected with HA-tagged A3H HapII and HapI, and the cell lystes were immunoprecipitated using anti-HA affinity matrix. Immunoprecipitated RNA were purified and reverse transcribed, and the relative amounts of 7SL, Y RNAs and GAPDH were measured. B) Homology modeling of A3H HapII showing that 15N lies close to the putative RLYYHW RNA binding motif.