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. 2012 Jul 30;7(7):e41861. doi: 10.1371/journal.pone.0041861

Figure 3. Analysis of several identified proteins.

Figure 3

The readout of the DeCyder Biological Variation Analysis (BVA) module is shown for ATP synthase subunit E (ATPS, spot 11), putive glutamine synthetase (GS, spot No. 14), 26S proteasome regulatory subunit (26S PRS, spot No. 20), ascorbate peroxidase (APX, spot No. 26), 14-3-3 protein (14-3-3, spot No. 34) and chalcone synthase family protein (CHS, spot No. 37). Enlarged regions of 2D-DIGE gels for Cy3-labeled WT (green) and Cy5-labeled YX-1 (red), and the corresponding 3D views, are represented. The bottom panel shows a graphic representation of the differences in abundance of these proteins across three independent experiments. For normalization purposes, a Cy2-labeled internal standard was included, corresponding to a pool of protein from all extracts used in the analysis (st, standard).