. 2012 Jul 30;7(7):e41861. doi: 10.1371/journal.pone.0041861

Table 2. Proteins from WT and YX-1 anthers of wolfberry analyzed by DIGE and MALDI-TOF/TOF, and identified by searching against NCBInr and Viridiplantae EST databases.

^aSpot No.	^bAcc. No.	^cProtein name (Species)	^dMr/pI	^eScore	^fS.C. (%)	^gS.V.R. (YX-1/WT)	^h p-value
Photosynthesis-related
1	gi\|170320	ribulose-1,5-bisphosphate carboxylase (NS)	10.3/5.37	100	80	−1.57	0.0083
2	gi\|445628	RuBisCO activase(NT)	42.9/5.52	100	85	−2.76	0.0013
3	gi\|230922	unactivated form Of ribulose-1,5- bisphosphatecarboxylase(NT)	14.7/5.19	100	80	−2.20	0.0048
4	gi\|170320	ribulose-1,5-bisphosphate carboxylase (NS)	10.3/5.37	100	77	−1.70	0.00015
Carbohydrate and energy metabolism
5	gi\|1781348	homologous to plastidic aldolases (ST)	38.6/5.89	100	17	−1.65	0.0011
6	gi\|4539543	glyceraldehyde-3-phosphate dehydrogenase (NT)	36.8/7.7	100	42	−2.52	0.00024
7	gi\|4539543	glyceraldehyde-3-phosphate dehydrogenase (NT)	36.8/7.7	100	42	−2.38	0.0066
8	gi\|21592495	fructokinase-like protein (AT)	35.2/5.12	100	27	−2.65	0.0027
9	gi\|21388550	malate dehydrogenase(ST)	36.4/8.48	100	22	−1.88	0.0076
10	gi\|48209968	ATP synthase D chain, mitochondrial (ST)	29.8/5.34	100	60	−2.61	0.0011
11	gi\|9652289	putative ATP synthase subunit E (SL)	27.4/6.63	100	47	−2.73	0.00024
12	gi\|56784991	putative ATP synthase beta subunit (OS)	45.9/5.33	100	22	−1.72	0.00078
Nucleic acid metabolism
13	gi\|8272416	nucleoside diphosphate kinase 3(BR)	21.5/7.98	100	36	−1.83	0.0012
Amino acid metabolism
14	gi\|28393681	putative glutamine synthetase(AT)	38.9/5.59	97.2	14	−2.97	0.00023
15	gi\|18414289	ARD(AT)	23.5/4.99	99.9	14	−2.48	0.0033
16	gi\|4049354	glycine hydroxymethyltransferase-like protein (AT)	50.9/8.13	100	22	−2.67	0.0052
Protein metabolism
17	gi\|19851	cysteine protease (NT)	40.8/6.0	96.2	26	−2.42	0.0017
18	gi\|415833	5B protein (SL)	11.6/8.16	100	29	−1.95	0.00005
19	gi\|37805883	putative S-phase Kinase association Protein 1(SKP1) (OS)	35.5/5.57	98.6	9	2.42	0.0039
20	gi\|18424049	26S proteasome regulatory subunit (AT)	24.4/5.08	99.5	27	2.59	0.0054
21	gi\|30693656	ubiquitin-protein ligase (AT)	16.8/6.2	100	46	−2.89	0.0024
^*22	gi\|309372357	NT2C-EST-0809 (NT) similar to aspartic protease gi\|226503984(ZM)	18.7/6.64	99.9	10	2.23	0.011
23	gi\|20137686	cysteine protease inhibitor 5(ST)	17.1/8.63	98	16	2.10	0.0052
24	gi\|30692346	ribosomal protein S1(AT)	45.3/5.13	100	19	−2.88	0.00026
25	gi\|1848212	protein disulfide-isomerase (NT)	40.1/5.99	100	14	2.74	0.0089
Stress related
26	gi\|34809902	ascorbate peroxidase(NT)	32.3/5.96	98.7	15	−2.76	0.0094
27	gi\|34809902	ascorbate peroxidase(NT)	32.3/5.96	100	26	−1.53	0.0048
28	gi\|21039134	ascorbate peroxidase (SL)	42.4/8.65	100	26	−2.44	0.0021
29	gi\|55296784	putative peroxidase (OS)	35.4/6.43	95.1	25	−2.34	0.002
Transcription factor
30	gi\|15220876	putative transcription factor BTF3 (AT)	17.9/6.62	100	30	−2.17	0.00017
Signaling-related
31	gi\|9979177	translationally-controlled tumor protein(NT)	18.9/4.54	98.9	20	2.65	0.000038
32	gi\|75319566	calmodulin-like protein 1(OS)	21.1/4.75	100	31	−1.77	0.006
33	gi\|15233402	putative calcium-binding protein (AT)	21.2/4.59	100	40	−1.79	0.000012
34	gi\|3766535	14-3-3 protein (ST)	29.4/4.78	100	58	−1.99	0.00035
Anther development
35	gi\|4588012	putative callose synthase catalytic subunit (GH)	21.9/8.42	100	27	−2.43	0.00067
36	gi\|2326772	chalcone synthase -like protein(NS)	40.7/5.59	100	30	−2.59	0.0011
Flavonoid synthesis
37	gi\|15217605	chalcone synthase family protein (AT)	43.9/6.01	100	20	−2.78	0.0047
Fatty acid synthesis
38	gi\|2204236	enoyl-ACP reductase(NT)	33.9/6.41	99.9	8	−2.32	0.0003
Unknown proteins
39	gi\|115474835	Os08g0154300 (OS)	43.1/5.18	100	18	−1.87	0.0016
40	gi\|2673909	hypothetical protein (AT)	13.1/4.59	99	18	2.24	0.00073
41	gi\|54291158	hypothetical protein (OS)	14.0/7.7	99.8	21	2.43	0.0015
Others
42	gi\|56562181	formate dehydrogenase (SL)	42.4/6.87	100	30	2.54	0.00022
43	gi\|15228869	copper chaperone (AT)	13.1/4.91	99.8	30	−2.96	0.0061
44	gi\|15228869	copper chaperone (AT)	13.1/4.91	99.9	26	−1.50	0.0032
45	gi\|30580343	caffeoyl-CoA O-methyltransferase 6(NT)	27.9/5.3	100	32	2.33	0.00022

Spot number in 2-DE gel, as shown in Fig. 2.

Accession number in NCBInr/EST database.

Protein names and species from the NCBInr/EST database. NT, Nicotiana tabacum; NS, Nicotiana sylvestris; AT, Arabidopsis thaliana; SL, Solanum lycopersicum; ST, Solanum tuberosum; BR, Brassica rapa; ZM, Zea mays; OS, Oryza sativa.GH, Gossypium hirsutum.

Theoretical molecular weight and pI of the identified proteins.

Mascot protein score for ions complemented by the percentage of the confidence index (C.I.).

Sequence Coverage.

Spot volume ratios are the average for each spot from three replicate gels.

Protein spots with a significant change in abundance (1.5-fold or above) between the WT and YX-1, and a P-value of≤0.05 were considered statistically significant.

Spots from EST database.

Unidentified differently spots not listed.