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. 2012 Jul 30;7(7):e41414. doi: 10.1371/journal.pone.0041414

Figure 4. Active SLE plasma induce the expression of IFN-I dependent genes.

Figure 4

PBMC from three healthy donors were individually stimulated for 1 h with plasma from obtained from 5 inactive SLE, 5 active SLE patients, 3 healthy controls or 50 U/ml recombinant IFNαβ. Induction of the IFN-I dependent genes 2′5′OAS (A), MxA (B) or EIF2α (C) was examined by qRT-PCR (SYBR-Green). Graphs show the induction on PBMC from three healthy donors (red, yellow and blue circles indicate different donors) for the indicated gene, under the indicated conditions for each column (control plasma, 50 U/ml IFNββ and plasma from inactive or active SLE). Data are expressed as the x-fold induction of each mRNA compared to unstimulated PBMC. D, E and F: Pearson’s correlation between induction of each gene (mean of PBMC from three healthy donors) and SLEDAI scores. D = 2′5′OAS, E = MxA, F = EIF2α. G: Western blot analysis of one SLE patient (left) and one healthy control (right)-PBMC lysates cultured for 1 hour with increasing concentrations of IFNβ (0, 1.5, 5, 15 and 50 U/ml, blotted with anti-pJak1 antibody.