Figure 1. Single-step selection method.

FAM-labeled oligonucleotide library containing a 40 nt random region was incubated with a target immobilized on a glass coverslip. Unbound sequences were discarded by extensive washing followed by fluorescence microscopy. The coverslip was later crushed and eluted the bound sequences by heating in water. The selected aptamers were amplified by PCR and cloned into E.coli and the purified individual plasmid DNA was sequenced.