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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Histochem Cell Biol. 2012 May 15;138(2):231–241. doi: 10.1007/s00418-012-0961-0

Fig. 4. Western blot analysis of mouse atrial tissues.

Fig. 4

For determination of the amount of Cav-1, Cav-3 and P2X7R, cell homogenates of atrium from wild-type and cav-1−/− mice were prepared and subjected to western blot analysis. 50 #g of protein from each sample was loaded on the gel. Western blot analysis was performed using rabbit anti-P2X7R antibody (dilution 1:500), rabbit anti-Cav-1 antibody (dilution 1:500), mouse anti-Cav-3 antibody (dilution 1:500) and anti-γ-tubulin antibody (1:1000). γ-tubulin served as a loading control. Representative data from 5 separate experiments are shown.