Skip to main content
. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jul 5;2(1):89–100. doi: 10.1016/j.celrep.2012.05.018

Figure 4.

Figure 4

Vesicles carrying TfR-GFP-FM4 behave differently from those carrying FM4-VSVGΔC-GFP or FM4-NgCAM-GFP in axons, but similarly in dendrites (see also Figure S3 and Movies, 4, 5). (A) Distance traveled versus time plots for vesicles carrying TfR-GFP-FM4 in the axon reveal that most vesicles did not move more than 10 μm beyond the cell body following release from the Golgi. In dramatic contrast, similar plots for vesicles carrying FM4-VSVGΔC-GFP (B) reveal that these vesicles proceeded up to 60 μm into the axon. Similarly, plots for vesicles carrying FM4-NgCAM-GFP (C) reveal that these vesicles proceeded up to 50 μm into the axon. (D, E, F) Plots of distance traveled versus time for vesicles containing TfR-GFP-FM4 or FM4-VSVGΔC-GFP or FM4-NgCAM-GFP in dendrites indicate that all three types of vesicles behave in a similar manner in this compartment.( G, H) Axon and dendrite scatter plots showing the ratio of the number of vesicles that proceed versus the number that halt or reverse for individual cells. Note that each data point refers to a single cell. Vesicles carrying TfR-GFP-FM4 behave differently from those carrying FM-VSVGΔC-GFP or FM4-NgCAM-GFP in axons, but similarly in dendrites.