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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jul 12;2(1):136–149. doi: 10.1016/j.celrep.2012.06.005

Figure 1. Detection of oxidatively modified proteins in DC purified from primary and secondary lymphatic organs.

Figure 1

a,b,c,d,e) Western blot analysis of carbonylated proteins detected in a) purified CD34+ bone marrow precursors b) bone marrow dendritic cells (BMDC) cultured in GM-CSF for 7 days and c,d,e) conventional CD11c+ DC freshly purified from primary and secondary lymphatic organs from 3, 12, and 22 month old mice. One representative experiment out of four is shown. f) Western blot analysis of lipoxidated proteins (probing for malondialdehyde) detected in conventional CD11c+ freshly purified splenic DC. Lanes marked as (d) report derivatized proteins and (nd) report non-derivatized proteins (specificity control). Loading controls are reported in Supplement Figure 1. g) Pie chart reporting the subcellular distribution of the oxidative proteome immunoprecipitate from conventional CD11c+ splenic DC purified from 22 month old mice.