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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Thromb Haemost. 2011 Dec 8;107(1):111–123. doi: 10.1160/TH11-10-0685

Fig. 5. Triplatin does not interact with GPVI and its activity is loss in the presence of indometacin.

Fig. 5

(A) Convulxin (10 nM), triplatin (1 μM), or DPTL (1 μM) was injected for 180 sec over immobilized GPVI using HBSP as a buffer. Dissociation was carried out for 5 min followed by regeneration of CM5 chip with HCl (10 mM). As a control, Cvx was injected again without loss of interaction with GPVI. (B) Calcein-labeled human platelets (2 × 105/μl) were incubated with fibrillar or soluble collagen for 1 h at indicated concentrations of triplatin. EDTA was used to discriminate GPVI- or integrin α2β1-mediated adhesion. Absolute fluorescence values are reported. Platelet adhesion BSA-blocked wells was negligible (n=6). (C) platelet-rich plasma (2 × 105/μl) was incubated with 50 μM indometacin, or 0.2 μM SQ29548 for 3 min alone, or with and without triplatin (1 μM) followed by addition of collagen (6.6 μg/ml).