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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jul 20;2(1):198–205. doi: 10.1016/j.celrep.2012.06.019

Figure 2. Introduction of a DNA affinity handle for purification of a specific chromosome section.

Figure 2

(A) S. cerevisiae strain LEXA::GAL1 pLexA-PrA was created by insertion of a LEXA DNA binding site upstream of the GAL1 start codon via homologous recombination. The pLexA-PrA plasmid was introduced into this strain and the constitutive expression of the LexA-PrA fusion protein was confirmed by western blotting for PrA. (B) Introduction of the LEXA DNA binding site does not impede GAL1 transcription. cDNA from wild type or LEXA::GAL1 pLexA-PrA strains grown in glucose or galactose was used as a template for real time PCR analysis of GAL1 vs ACT1 gene transcription. Error bars are the standard deviation of triplicate analyses.