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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jul 20;2(1):198–205. doi: 10.1016/j.celrep.2012.06.019

Figure 3. Efficiency of GAL1 chromatin purification.

Figure 3

(A) The effect of buffer stringency on purification of LexA-PrA with associated chromatin was evaluated with ChIP. Strain LEXA::GAL1 pLexA-PrA was subjected to ChIP using the following buffer with the reagents indicated on the graph: 20 mM hepes, pH 7.4, 0.1% tween-20, and 2 mM MgCl2. Enrichment of GAL1 DNA relative to ACT1 DNA was monitored by real-time PCR. (B) ChIP was used to measure the specificity of enrichment of LexA-PrA bound chromatin. Enrichment was monitored by real-time PCR with primer sets at the indicated chromosomal locations. (C) GAL1 chromatin is enriched in both glucose and galactose growth conditions. The relative efficiency of GAL1 enrichment was monitored by real-time PCR with primers targeted to the “0” position in panel B and to ACT1. The standard error is indicated.