Skip to main content
. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jul 20;2(1):198–205. doi: 10.1016/j.celrep.2012.06.019

Figure 4. ChAP-MS analysis of GAL1 chromatin.

Figure 4

(A) Enrichment of GAL1 chromatin under transcriptionally repressive glucose and active galactose growth conditions. Strain LEXA::GAL1 pLexA-PrA was grown in either glucose or galactose and subjected to affinity purification of GAL1 chromatin via LexA-PrA as detailed in Figure 1. Addition of an equivalent amount of isotopically heavy (13C615N2-lysine) cells lacking the LexA DNA binding site provided for the identification of proteins specifically enriched with GAL1 chromatin. Proteins co-enriching with LexA-PrA were resolved by SDS-PAGE and visualized by Coomassie-staining. Each gel lane was sliced into 2 mm sections. Gel slices were treated with trypsin and resulting peptides were analyzed by high resolution mass spectrometry. (B) Representative high resolution mass spectra from proteins and histone posttranslational modifications identified from the purification of transcriptionally active GAL1 chromatin.