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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jul 20;2(1):198–205. doi: 10.1016/j.celrep.2012.06.019

Figure 6. Validation of proteins and histone posttranslational modifications on GAL1 chromatin.

Figure 6

ChIP was targeted to Gal3-TAP, Spt16-TAP, Rpb2-TAP, H3K14ac and H3K36me3 under transcriptionally active galactose and repressive glucose growth conditions. ChIP to general H3 was used as a nucleosome occupancy control for each histone posttranslational modification ChIP. Enrichment of the 5’-end of GAL1 DNA relative to ACT1 DNA was monitored by real-time PCR. The standard error is indicated.