(A) Targeting strategy for importin β1 3′UTR. The loxP insertion sites are marked in red and the location of the 3′ and 5′ homology arms are in black. The PGK-neo selection cassette is inserted downstream of the region to be deleted (orange arrows) and flanked by FRT sites (green) that can be deleted using FLP recombinase. Three SV40 polyA signals are inserted immediately downstream of the floxed region (yellow boxes). For full sequences of the targeting construct and recombined locus please see Supplementary Procedures.
(B) Deep sequencing analyses on RNA from knockout and wild type DRG confirms deletion of the targeted 3′UTR region in PGK-Cre/Importin β1 3′UTR loxP mice.
(C) RT-PCR on RNA from knockout brain, DRG and sciatic nerve confirms complete deletion of the targeted 3′UTR region in PGK-Cre/Importin β1 3′UTR loxP mice.