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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Br J Haematol. 2011 Jan 12;152(4):469–484. doi: 10.1111/j.1365-2141.2010.08548.x

Fig 7.

Fig 7

AHR knockdown results in increased DNA synthesis of low ploidy cells without influencing apoptosis of differentiating CHRF cells. BrdU incorporation was used to investigate the role of AHR down regulation on the cell cycle of PMA-differentiated NegA, AHR-A and AHR-B cells (n = 3 for each cell line). (A) Representative experiments after 5 d of PMA-induced differentiation and their (B) corresponding ploidy histograms. Dashed lines indicate high ploidy (≥8n) cells; percent polyploidization (P2 + P4) is indicated. (C) BrdU incorporation (P1 + P2) was analyzed during PMA-induced differentiation of NegA (black squares), AHR-A (open triangles) and AHR-B (open circles) cells. (D) A further analysis of the data investigated the percentage of BrdU+ cells that were polyploid [P2/(P1 + P2)]. (E) Caspase-3 activity was assessed as a measure of apoptosis in differentiating CHRF cell lines. Error bars represent the SEM.