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. Author manuscript; available in PMC: 2012 Jul 31.
Published in final edited form as: Cell Rep. 2012 Jan 26;1(1):36–42. doi: 10.1016/j.celrep.2011.10.003

Table 1.

Segregation of heterozygous mutations in the Mut4 line

locus location knockout recessive lethal? WT:mutant allele
KIN82 Chr3 275085 no 3:3
EPLI Chr6 88632 no 5:11
PHO4 Chr6 225029 no 4:2
YBR219C Chr2 662320 no 3:3
AIM19 Chr9 199795 no 2:4
TOM70 Chr14 399797 no 2:4
AVT4 Chr14 435396 no 2:4
BIO3 Chr14 734316 no 2:4
FMP27 Chr12 1047541 no 2:4
ERV41 Chr13 139505 no 4:7
BUL1 Chr13 816264 no 4:2
YJRO12C Chr10 460308 no 3:5
KRR1 Chr3 22745 yes 12:0
KRS1 Chr4 525612 yes 12:0
CDC7* Chr4 424584 yes 10:2
RAD3 Chr5 528691 yes 12:0
MDN1 Chr12 363531 yes 16:0
FMP40 Chr16 131383 no 4:2
RAD1 Chr16 509432 no 2:9
REC8 Chr16 569931 no 1:10
Chr16 639362 no 1:11
RRP15 Chr16 818766 yes 12:0

Spore clones obtained from tetrads dissected from Mut4 at generation 160 were genotyped by Sanger sequencing as described in the Supplemental Experimental Procedures.

*

non-synonymous mutation.