Table 1.
Segregation of heterozygous mutations in the Mut4 line
| locus | location | knockout recessive lethal? | WT:mutant allele |
|---|---|---|---|
| KIN82 | Chr3 275085 | no | 3:3 |
| EPLI | Chr6 88632 | no | 5:11 |
| PHO4 | Chr6 225029 | no | 4:2 |
| YBR219C | Chr2 662320 | no | 3:3 |
| AIM19 | Chr9 199795 | no | 2:4 |
| TOM70 | Chr14 399797 | no | 2:4 |
| AVT4 | Chr14 435396 | no | 2:4 |
| BIO3 | Chr14 734316 | no | 2:4 |
| FMP27 | Chr12 1047541 | no | 2:4 |
| ERV41 | Chr13 139505 | no | 4:7 |
| BUL1 | Chr13 816264 | no | 4:2 |
| YJRO12C | Chr10 460308 | no | 3:5 |
| KRR1 | Chr3 22745 | yes | 12:0 |
| KRS1 | Chr4 525612 | yes | 12:0 |
| CDC7* | Chr4 424584 | yes | 10:2 |
| RAD3 | Chr5 528691 | yes | 12:0 |
| MDN1 | Chr12 363531 | yes | 16:0 |
| FMP40 | Chr16 131383 | no | 4:2 |
| RAD1 | Chr16 509432 | no | 2:9 |
| REC8 | Chr16 569931 | no | 1:10 |
| Chr16 639362 | no | 1:11 | |
| RRP15 | Chr16 818766 | yes | 12:0 |
Spore clones obtained from tetrads dissected from Mut4 at generation 160 were genotyped by Sanger sequencing as described in the Supplemental Experimental Procedures.
*
non-synonymous mutation.