Figure 1.

Representative MMA output. Each row represents the results from a single multiplex PCR amplification hybridized onto 12 target-specific probes in a single reaction. The resulting MFI signals generated by each probe-bound PCR product are shown for three control samples, a total RNA sample isolated from a translocation-negative cell line (HL60), 12 different synthetic fusion transcripts prepared by in vitro transcription and spiked in a background of HL60 RNA (400 ng input) and eight total RNA samples purified from translocation-positive cell lines (400 ng input). Target-specific positive signals above the qualitative cutoff (350 MFI) are highlighted. The three controls are designed to assess the validity of the multiplex amplification, hybridization and detection steps in every batch/run.