Table 2.
Fatty acid profiles of Arabidopsis thaliana T3 homozygous seeds transformed with amiRNAs targeting FAE1 (pJ1109) and FATB (pJ1110), respectively.
| Fatty acids | Control | Silenced gene |
|
|---|---|---|---|
| Columbia | FAE1 | FAT-B | |
| 16:0 | 8.0 ± 0.3 | 8.0 ± 0.2 | 4.4 ± 0.5 |
| 16:1Δ9 | 0.3 ± 0.1 | 0.1 ± 0.0 | 0.1 ± 0.1 |
| 18:0 | 3.1 ± 0.2 | 2.9 ± 0.3 | 2.0 ± 0.1 |
| 18:1Δ9 | 12.9 ± 0.1 | 24.0 ± 0.8 | 16.0 ± 0.8 |
| 18:1Δ11 | 1.9 ± 0.1 | 2.2 ± 0.1 | 1.6 ± 0.1 |
| 18:2Δ9,12 | 31.4 ± 0.6 | 34.9 ± 1.1 | 32.5 ± 0.5 |
| 18:3Δ9,12,15 | 19.6 ± 0.4 | 25.1 ± 0.9 | 23 ± 0.7 |
| 20:0 | 2.0 ± 0.0 | 0.4 ± 0.1 | 1.0 ± 0.1 |
| 20:1Δ11 | 15.4 ± 0.3 | 1.9 ± 1.0 | 15.2 ± 0.6 |
| 20:1Δ13 | 1.7 ± 0.1 | 0.4 ± 0.1 | 1.5 ± 0.2 |
| 20:2Δ9,12 | 1.8 ± 0.1 | 0.1 ± 0.0 | 1.4 ± 0.1 |
| 22:0 | 0.5 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.0 |
| 22:1Δ13 | 1.3 ± 0.1 | – | 1.2 ± 1.2 |
| 24:0 | 0.1 ± 0.0 | – | – |
Values are the means of the entire T3 seed of 21 plants (% of total fatty acids) with the errors reported as SD. Fatty acids directly affected by the gene silencing are shown in bold.