Table 3.
Fatty acid profiles of Arabidopsis thaliana T4 homozygous seeds transformed with single-gene amiRNAs (pJP1106) targeting FAD2.
| Fatty acids | Control Columbia | FAD2 silenced line | hpRNA silenced line (3-1-1) |
|---|---|---|---|
| 14:0 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 16:0 | 6.8 ± 0.1 | 4.7 ± 0.1 | 4.9 ± 0.2 |
| 16:1Δ9 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.40 ± 0.0 |
| 16:3Δ9,12,15 | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 18:0 | 2.9 ± 0.0 | 2.8 ± 0.1 | 2.8 ± 0.1 |
| 18:1Δ9 | 18.3 ± 1.0 | 63.3 ± 1.1 | 58.1 ± 1.1 |
| 18:1Δ11 | 1.7 ± 0.1 | 2.1 ± 0.0 | 2.1 ± 0.2 |
| 18:2Δ9,12 | 27.5 ± 0.2 | 1.7 ± 0.2 | 3.3 ± 0.1 |
| 18:3Δ9,12,15 | 17.3 ± 0.9 | 3.0 ± 0.2 | 5.0 ± 0.1 |
| 20:1Δ11 | 17.6 ± 0.3 | 17.5 ± 0.7 | 18.5 ± 1.1 |
| 20:1Δ13 | 1.8 ± 0.1 | 1.7 ± 0.1 | 1.8 ± 0.1 |
| 20:2Δ11,14 | 1.6 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| 20:3Δ11,14,17 | 0.4 ± 0.0 | 0.0 ± 0.0 | 0.1 ± 0.0 |
| 20:0 | 1.7 ± 0.1 | 1.1 ± 0.2 | 1.1 ± 0.7 |
| 22:0 | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.3 ± 0.0 |
| 22:1Δ13 | 1.5 ± 0.1 | 0.8 ± 0.1 | 0.9 ± 0.1 |
| 24:0 | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 |
| 24:1Δ15 | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.2 ± 0.0 |
Control Columbia and FAD-2-HP silenced line (Stoutjesdijk et al., 2002) profile were also shown. Values are the means of the entire T4 seeds of 23 independent plants (% of total fatty acids) with the errors reported as SD. Fatty acids directly affected by the gene silencing are shown in bold.