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. Author manuscript; available in PMC: 2012 Oct 27.
Published in final edited form as: Neuroscience. 2011 Aug 4;194:220–226. doi: 10.1016/j.neuroscience.2011.07.072

Figure 2.

Figure 2

Secreted and intracellular GDNF in primary ventral midbrain cultures transfected with pGDNF plasmids. Ventral midbrain cultures were established as described in the Methods section and transfected with one of the following plasmids: pGDNF_1a (1a), pGDNF_1b (1b), pGDNF_2a (2a), pGDNF_2b (2b), pGDNF_3a (3a), or pGDNF_3b (3b). Each bar represents the average (± s.e.m.; n=5) amount of GDNF protein detected in cells or secreted in the culture media. *p<0.05, 1b vs. 1a, 2a, 3a, 2b, 3b; ^p<0.05 2b vs. 3a, 2a; #p<0.05 1b vs 2a, 3a, 3b.