Figure 2. Short-term characterization of marker expression in FACS-purified cells.

(A) qRT-PCR data showing enrichment of Nurr1 (14.6 fold) and Pitx3 (10.0 fold) in reporter⁺ cells compared with that in reporter^– cells of the respective cell lines. (B–K) Immuno­cyto­chemical analysis of reporter⁺ cells 24 hours after sorting. (B) Quantitative analysis in Hes5, Nurr1, and Pitx3 lines for coexpression of GFP and TH (GFP⁺/TH⁺, TH⁺/GFP⁺), the percentages of GFP⁺, Tuj1⁺, and TH⁺ cells among total cells (GFP⁺/DAPI⁺, Tuj1⁺/DAPI⁺, TH⁺/DAPI⁺), and the colocalization of midbrain markers EN1, LMX1A, and FOXA2 in TH⁺ cells (EN1⁺/TH⁺, LMX1A⁺/TH⁺, FOXA2⁺/TH⁺). (C–K) Representative images of immuno­cyto­chemical marker analysis. Scale bar: 50 μm.