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. 2012 Jul 2;122(8):2928–2939. doi: 10.1172/JCI58767

Figure 5. Microarray analysis of FACS-purified cell populations.

Figure 5

(A) Venn diagram plot for significantly enriched (increased expression only) genes in intraline analysis, showing 107 genes common to both Nurr1::GFP+ and Pitx3::YFP+. (B) Venn diagram plot of significantly enriched genes in interline analysis, showing 148 genes common to both Nurr1::GFP+ and Pitx3::YFP+ cells in contrast to Hes5::GFP+ cells. (C–E) Scatter plots of significantly altered genes (≥2 fold increased/decreased; P ≤ 0.05). Red boxes represent genes enriched at the Hes5 stage (H+/H–), while blue boxes represent genes that are decreased at that stage. (C) Intraline scatter plot of the 233 altered H+/H– genes (x axis) compared with the altered 232 N+/N– genes (y axis) (449 genes common to both). (D) Intraline scatter plot of the 232 altered N+/N– genes compared with the 556 altered P+/P– genes (656 genes common to both). (E) Interline scatter plot of the 586 altered N+/H+ genes compared with the 1,203 altered P+/H+ genes (1,355 genes common to both). (F) Dendrogram plot of all significantly altered genes (P ≤ 0.05) in all 3 reporter+ and reporter– sorts across the 3 lines. H, Hes5; P, Pitx3; N, Nurr1.