Figure 2. Prolyl hydroxylase inhibitor DMOG specifically inhibits the ATR/CHK1/p53 pathway following DNA damage.

(A) A simplified schematic of the signaling pathways induced by DNA damage. (B) HeLa cells were pretreated with DMOG for 4 hours, followed by NCS treatment as indicated. Western blots were performed with indicated antibodies. (C) HeLa cells were pretreated with DMOG for 4 hours followed by UV treatment at different doses. Cells were harvested after 2 hours, and Western blots were performed with the indicated antibodies. (D–F) HeLa cells were pretreated with DMOG for 4 hours, followed by UV treatment (250 j/m²), and harvested at different time points as indicated. (D) Western blots were performed with the indicated antibodies. TUNEL staining was performed at 8 hours after UV treatment. Representative images of cells pretreated with PBS or DMOG are shown in E. Scale bars: 20 μm. Original magnification, ×100. (F) TUNEL-positive cells (mean ± SEM) were quantified. n = 3; *P < 0.01. In B–D, representative blots from 3 similar experiments are shown.