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. 2012 Jul 17;122(8):2827–2836. doi: 10.1172/JCI62374

Figure 3. PHD3 plays a crucial role in DDR.

Figure 3

(A) HeLa cells transfected with si-scramble or si-PHD3 for 3 days were treated with NCS for 2 hours. si-PHD3-1 and si-PHD3-2, si-RNA specific for PHD3 construct 1 and 2, respectively. Cell lysates were analyzed by Western blot with the indicated antibodies. Representative blots from 2 experiments are shown. (B and C) HeLa cells transfected with si-scramble or si-PHD3 for 3 days were immunostained with anti-RPA32 antibody. Representative images are shown in B. The bottom panel in B shows magnified images of the white boxes in the middle panel. Scale bars: 10 μm. Original magnification, ×200. (C) Quantification of the number of cells positive for RPA32 (mean ± SEM) from 3 independent experiments. *P < 0.01.