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. 2012 Jul 17;122(8):2827–2836. doi: 10.1172/JCI62374

Figure 4. DMOG inhibits the interaction between HCLK2 and ATR but not ATR activity.

Figure 4

(A) An in vitro kinase assay was performed with GST-p531–47 and wild-type ATR or mutant ATRK2327R lacking the kinase activity immunoprecipitated from HEK293 cells in the presence or absence of DMOG. Western blots were then performed with the indicated antibodies. Representative blots from 3 similar experiments are shown. (B) After 6 hours of treatment with DMOG, HeLa cells transfected with pcDNA3 or Flag-HCLK2 were harvested and immunoprecipitated using anti-Flag beads. Western blots were then performed with the indicated antibodies. Densitometry analysis (mean ± SEM) was performed for the coimmunoprecipitated ATR. n = 3; *P < 0.01.