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. 2012 Jul 2;122(8):2807–2816. doi: 10.1172/JCI63563

Figure 1. Sortilin demonstrates high-affinity binding to APOB.

Figure 1

(A) Preparations of LDL at the indicated concentrations in HBS-EP buffer (pH 7.4) were passed across the sensor chip for 240 seconds to monitor the association of LDL, HDL, and APOE DMPC discs and sortilin, and then the chip was exposed to buffer alone for an additional 240 seconds to monitor the dissociation phase. (B) 100 μg/μl of LDL was bound to immobilized human sortilin in HBS-EP buffer at pH 7.4. At the indicated time point, the buffer was exchanged with HBS-EP at lower pH values.