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. 2012 Jul 17;122(8):2989–3001. doi: 10.1172/JCI64427

Figure 2. Increased retinal autofluorescence in A/J mice with age relates to inflammatory changes and immune cell infiltration.

Figure 2

(A) Representative SLO autofluorescent images of the outer retina (480 nm excitation; emission filter, 500–700 nm) of 1- and 8-month-old A/J and B6 mice. A/J mice exhibited increased autofluorescent spots with age, which were negligible in B6 mice (n > 3). ND, not detectable. (B) SD-OCT imaging of A/J and B6 mice at 1 and 8 months of age revealed infiltrating cells only in the subretinal space of 8-month-old A/J mice (left; red arrows) that were also observed in plastic block sections (right; black arrows). Thickness of ONL in OCT images is indicated by arrows to highlight the decline in A/J versus B6 mice at 8 months. (C) The cellular infiltration seen by OCT and in plastic sections was of inflammatory origin, as evidenced by increased Iba-1 staining (activated microglia cells) and increased GFAP staining (activated Müller glial cells and astrocytes) in retinas of 8- versus 1-month-old A/J mice. These age-related changes were absent from 8-month-old B6 mouse retina. GCL, ganglion cell layer; IPL, inner plexiform layer; PR, photoreceptor layer. Scale bars: 50 μm.