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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Exp Cell Res. 2012 Jun 13;318(16):2094–2104. doi: 10.1016/j.yexcr.2012.05.017

Figure 2.

Figure 2

Effects of Olo II on cell cycle progression. A, The effect of Olo II on the cell cycle profiles at different time points. DBA252 cells were treated with 10 μM Olo II for the time points indicated and the cell cycle profile was analyzed by flow cytometry. B & C, DBA252 cells were synchronized by incubation with 50 ng/ml nocodazole for 16 h to arrest the cells at the G2M phases (Noc). Nocodazole was washed out with fresh medium and incubated for 1 h to allow cells to reenter the cell cycle (designated as 0 h). Afterward, the cells were cultured in the absence of (B) or in the presence of 10 μM Olo II (C) for the indicated time courses. The cells were collected at different time points and analyzed for cell cycle progression by flow cytometry.