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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Exp Cell Res. 2012 Jun 13;318(16):2094–2104. doi: 10.1016/j.yexcr.2012.05.017

Figure 3.

Figure 3

Effects of Olo II on the expression of cycle regulators. DBA 252 cells were treated under the conditions described in Fig.1. A, The mRNA levels of cyclins E, A, and B were determined by RT-qPCR. The mRNA level of tested gene in control cells (0 μM) is designated as 100%. The results are means ± SD of three independent experiments. Statistical analysis was performed with Student t test. The difference was considered statistically significant when p<0.05 (*). B, Graph, the protein levels of cyclin B determined by flow cytometry. The protein levels were determined by fluorescence intensity from a flow cytometer. The value determined from control cells (0 μM, control) is designated as 1. Inset, the protein levels of cyclin B was analyzed by Western blot. β-actin were used as a loading control.